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Just about every 24 h 20-40 mg of PtO2 was included as well as the development with the response monitored by 31P NMR spectroscopy. After elimination of the catalyst by filtration and the solvent by evaporation, the reliable residue was washed with ethyl ether to get N

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m is 1/four hundred of that measured with the wild style enzyme. These final results validate the importance with the role of H251 within the binding of substrate within the Energetic web-site of NagA. The homologous residue was uncovered to interact with d-glucosamine-six-phosphate within the framework of NagA from B. subtilis

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The second ionization observed during the log kcat/Km profile could originate from other residues inside the active internet site of NagA nevertheless it could also crop up from the protonation of your substrate by itself Considering that the phosphate moiety incorporates a p

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